Tools that we use

In our research we use both traditional and state of the art techniques to answer questions that are important to us. A list of our projects can be found here. Below you can find a description of main tools that we use or develop.

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immunofluorescence / brain clearing / whole brain mapping

In order to visualise the proteins at their location we use regular immunofluorescent staining as well as cutting edge brain clearing techniques. We collaborate with neuroinformaticians to map density of activated brain cells in whole brains of mice.

more in:
Bożycki Ł, Łukasiewicz K, Matryba P. et al Skeletal Muscle 2018 doi: 10.1186/s13395-018-0168-8.
Havekes R et al Elife 2016 doi: 10.7554/eLife.13424
Piechota M et al Oncotarget 2016 doi: 10.18632/oncotarget.12752
Radwanska K et al Neurobiol Learn Mem 2015 125:106-12
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Viral vectors

AAV, CAV, Lenti... you name it. Stereotactic injections of viral vectors into mous brain are a very common technique in the lab. These vectors allow to manipulate local protein expression and to express chemogenetic receptors to activate neurons of choice

more in:
Łukasiewicz K, Robacha M, et al J Vis Exp 2016 doi: 10.3791/53528
Beroun A, Nalberczak-Skóra M et al Neuropsychopharmacology 2018 doi: 10.1038/s41386-018-0119-4
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Behavioral assays / IntelliCage

In order to elucidate molecular basis of behavior we use a plethora of behavioral paragims. They consist of tratidional assays like fear conditioning and extinction paradigms or open field. Many of our experiments take place in Intellicages that allow for more ecologically-relevant assays, as mice are housed together and experimentor involvment is minimal.

more in:
Harda Z et al Genes Brain Behav 2018 doi: 10.1111/gbb.12457
Mijakowska Z, Łukasiewicz K, et al Addict Biol doi: 10.1111/adb.12327
Beroun A, Nalberczak-Skóra M et al Neuropsychopharmacology 2018 doi: 10.1038/s41386-018-0119-4
Stefaniuk M, Beroun A et al. Biol Psychiatry 2017 doi: 10.1016/j.biopsych.2016.12.026 (*equal contribution
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3D Electron Microscopy

To visualise synapses and dendritic spines we use 3D Electron Microscopy. Nencki Institue is equiped with a Serial Block-Face Scanning EM.

more in:
Borczyk M et al Scientific reports 2019 doi: 10.1038/s41598-018-38412-s
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In vitro work

To go into molecular details of synaptic plasticity we use both organotypic and dissociated hippocampals cultures.

more in:
Borczyk M et al Scientific reports 2019 doi: 10.1038/s41598-018-38412-s
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Electrophysiology

We have our own electrophysiology rig, where both field and patch-clamp recordings take place.

more in:
Beroun A, Nalberczak-Skóra M et al Neuropsychopharmacology 2018 doi: 10.1038/s41386-018-0119-4
Stefaniuk M, Beroun A et al. Biol Psychiatry 2017 doi: 10.1016/j.biopsych.2016.12.026